Th2 cytokines play a pivotal role in the pathogenesis of allergic rhinitis. To investigate the effect of p38 mitogen-activated protein kinase (MAPK) on the production of Th2 cytokines such as IL-4 and IL-5 in allergic rhinitis, a model of allergic rhinitis was established in SD rats. The expression level of p38 MAPK mRNA in PBMCs was detected by means of real time quantitative RT-PCR. The p38 MAPK activity in PBMCs was detected by Western blotting. PBMCs were cultured with various concentrations of p38 MAPK inhibitor SB 239063 or without the treatment, and then IL-4, IL-5 levels of the supernatant were determined by using sandwich ELISA. The results showed that mRNA expression and activity of p38 MAPK in PBMCs were significantly higher in allergic rhinitis rats than in control rats (P<0.05). The p38 MAPK inhibitor SB 239063 decreased the production of IL-4 and IL-5 in a dose-dependent manner. It is concluded that p38 MAPK plays an important role in the pathogenesis of allergic rhinitis which is associated with Th2 cytokines release.
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Otolaryngol Head & Neck Surg, Wuhan 430030, Peoples R China[2]Nanchang Univ, Affiliated Hosp 1, Dept Otolaryngol Head & Neck Surg, Nanchang 330006, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Liu Jie,Liu Lisi,Cui Yonghua,et al.p38 MAPK Regulates Th2 Cytokines Release in PBMCs in Allergic Rhinitis Rats[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES.2010,30(2):222-225.doi:10.1007/s11596-010-0218-x.
APA：
Liu, Jie,Liu, Lisi,Cui, Yonghua,Zhang, Jian&Jiang, Hongqun.(2010).p38 MAPK Regulates Th2 Cytokines Release in PBMCs in Allergic Rhinitis Rats.JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES,30,(2)
MLA：
Liu, Jie,et al."p38 MAPK Regulates Th2 Cytokines Release in PBMCs in Allergic Rhinitis Rats".JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES 30..2(2010):222-225
