单位：[1]Huazhong Univ Sci & Technol,Inst Organ Transplantat,Tongji Med Coll,Tongji Hosp,Wuhan 430030,Peoples R China器官移植研究所华中科技大学同济医学院附属同济医院器官移植[2]Minist Educ, Key Lab Organ Transplantat, Wuhan 430070, Peoples R China[3]Minist Hlth, Key Lab Organ Transplantat, Wuhan 430030, Peoples R China
A modified purification procedure is described for effectively eliminating dead cells after hepatocyte cryopreservation. Isolated hepatocytes from six pig tissue samples were cryopreserved Ill liquid nitrogen for 2 weeks After thawing, we developed a pre-incubation step prior to gradient centrifugation The hepatocytes were subsequent cultured in suspension overnight (12-16 h). and then dead cells were eliminated by Ficoll 400 Purification The results showed that a high viability (mean of 96%) of cells was obtained, with a low viable cell loss in number (2-5%), by using this modified method (C) 2009 Elsevier Inc All rights reserved
基金：
Major Scientific and Technological Project of Hubei Province [2007ABD005]
通讯机构：[1]Huazhong Univ Sci & Technol,Inst Organ Transplantat,Tongji Med Coll,Tongji Hosp,Wuhan 430030,Peoples R China[2]Minist Educ, Key Lab Organ Transplantat, Wuhan 430070, Peoples R China[3]Minist Hlth, Key Lab Organ Transplantat, Wuhan 430030, Peoples R China
推荐引用方式(GB/T 7714)：
Meng Fan-ying,Chen Zhi-shui,Han Meng,et al.An improved purification approach with high cell viability and low cell loss for cryopreserved hepatocytes[J].CRYOBIOLOGY.2010,60(2):238-239.doi:10.1016/j.cryobiol.2009.11.003.
APA：
Meng, Fan-ying,Chen, Zhi-shui,Han, Meng,Hu, Xin-peng&Zhou, Ping.(2010).An improved purification approach with high cell viability and low cell loss for cryopreserved hepatocytes.CRYOBIOLOGY,60,(2)
MLA：
Meng, Fan-ying,et al."An improved purification approach with high cell viability and low cell loss for cryopreserved hepatocytes".CRYOBIOLOGY 60..2(2010):238-239
