Anti-HER2 monoclonal antibody (Sc7301)-paclitaxel (TAX) immunoconjugate was prepared and its specific binding to tumor cells was investigated in this study. Sc7301 was conjugated to TAX by the active ester method and then the TAX-Sc7301 immunoconjugate was obtained. After purification and labeling by Cyano-fluorescein isothiocyanate (FITC), the specific binding of TAX-Sc7301 to HER2-positive tumor cells (SKOV3) and HER2-negative tumor cells (HepG2) was evaluated respectively. TAX-Sc7301 (20 nmol/L) showed distinct specific binding to SKOV3 cells rather than HepG2 cells. And the uptake of the immunoconjugate by SKOV3 cells was increased with the TAX-Sc7301 concentration (3-48 nmol/L) and the incubation time (P < 0.05). It was concluded that the TAX-Sc7301 immunoconjugate is potentially applicable as a targeted agent against HER2-positive tumor cells.
基金：
National Natural Science Foundation of China [30300429]
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Hosp, Dept Pharm, Tongji Med Coll, Wuhan 430030, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Liu Dong,Xu Yanjiao,Rao Zichao,et al.Preparation of anti-HER2 monoclonal antibody-paclitaxel immunoconjugate and its biological evaluation[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES.2011,31(6):735-740.doi:10.1007/s11596-011-0669-8.
APA：
Liu, Dong,Xu, Yanjiao,Rao, Zichao&Chen, Zhaocong.(2011).Preparation of anti-HER2 monoclonal antibody-paclitaxel immunoconjugate and its biological evaluation.JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES,31,(6)
MLA：
Liu, Dong,et al."Preparation of anti-HER2 monoclonal antibody-paclitaxel immunoconjugate and its biological evaluation".JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES 31..6(2011):735-740
