Background: Using condoms consistently could prevent unintended pregnancy among young people. This study highlights multiple domains of inuence on condom use among male college students in China, including knowledge, attitudes, health services utility on condom use and reproductive health information sources. Methodology/Principal Findings: To identify factors associated with condom use in Chinese male college students, we examined a sample of 870 sexually experienced male students in seven colleges in Wuhan, China, 2009. 535 (61.5%) of 870 male students reported condom use during their most recent sexual encounter. Male students with steady partners were more likely to use condoms than students with casual partners (adjusted OR = 3.11, 95%CI 2.30-4.20). And positive attitudes toward contraceptive responsibility were associated with greater odds of condom use (adjusted OR = 1.40, 95%CI 1.02-1.92). Only 54(6.2%) and 83(9.5%) of respondents reported that free condoms and reproductive health counseling were available at the student health center. Providing free condoms and reproductive health counseling at the student health central were associated with increased condom use among college students (both P<0.05). In addition, students who gained reproductive health information mainly through websites, television and radio programs were more likely to use condoms than through school education (all P<0.05). Conclusions: Improving attitudes of male students toward contraceptive responsibility, providing proper reproductive health information through mass media and making free condoms and reproductive health counseling available in school may help increase condom use among college students in China. Citation: Long L, Yuan T, Wang M, Xu C, Yin J, et al. (2012) Factors Associated with Condom Use among Male College Students in Wuhan, China. PLoS ONE 7(12): e51782. doi: 10.1371/journal.pone.0051782