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Activation of CD40 by soluble recombinant human CD40 ligand inhibits human glioma cells proliferation via nuclear factor-κB signaling pathway

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单位： [1]Huazhong Univ Sci & Technolgoy,Tongji Med Coll,Tongji Hosp,Dept Neurosurg,Wuhan 430030,Peoples R China [2]Huazhong Univ Sci & Technolgoy,Tongji Med Coll,Tongji Hosp,Dept Dermatol,Wuhan 430030,Peoples R China [3]5 Hosp Yichang City, Dept Neurosurg, Yichang 443000, Peoples R China

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关键词： CD40 human glioma cells NF-kappa B signaling pathway

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As CD40 transduces activation signals involved in inflammatory and immune disorders, we explored the expression and response to CD40 engagement in human glioma cell lines in this study. The CD40 expression in BT-325 and U251 cells was flow cytometrically detected. The cells were incubated with srhCD40L for 72 h to assess its effects on cell growth in vitro. TNF-alpha expression was quantified by real-time PCR, and protein expression was analyzed by ELISA. The I-kappa b mRNA was detected by RT-PCR. I-kappa B expression decreased after stimulation with 1 mu g/mL srhCD40L, but it was upregulated after the cells were pretreated with CD40 antibody. srhCD40L significantly inhibited the proliferation of the CD40+ human glioma cells. The stimulation of CD40+ glioma cells with soluble CD40L (CD154) up-regulated the expression of TNF-alpha at both mRNA and protein levels. We are led to conclude that CD40L/CD40 could inhibit human glioma cells through I-kappa b signaling pathway. Interferon-gamma can augment CD40 expression and the inhibitory effect of CD40 ligand on cell growth in vitro. These results suggest that srhCD40L may benefit the therapy strategy of glioma.

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出版当年[2011]版：

大类 | 4 区医学

小类 | 4 区生化与分子生物学

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出版当年[2010]版：

Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

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影响因子： 0 最新[2023版] 0 最新五年平均 0.405 出版当年[2010版] 0 出版当年五年平均 0.311 出版前一年[2009版] 0.385 出版后一年[2011版]

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第一作者单位： [2]Huazhong Univ Sci & Technolgoy,Tongji Med Coll,Tongji Hosp,Dept Dermatol,Wuhan 430030,Peoples R China

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