The aim was to assess the ability of a fast lactate dehydrogenase (LDH) staining technique to evaluate the boundary of the abnormal bowel segment in Hirschsprung's disease (HD) as a guide for surgical resection. Seventy children diagnosed with HD were equally divided into two groups. For the study group, fast LDH staining was used to confirm the diagnosis and determine the boundary of abnormal bowel. Frozen H&E staining was applied to the control group. Postoperatively, bowel samples were examined by paraffin H&E staining to confirm the intraoperative diagnosis. Patients received a follow-up analysis, and bowel function was scored and compared between the two groups. In the study group, 19 children were diagnosed with isolated HD, and the remaining had HD in combination with HD-allied disorders (HAD). The diagnosis was identical to the post-operative H&E staining, and the ganglia cells at the proximal end of the resected bowel were normal. In the control group, 30 children were diagnosed with isolated HD. However, the paraffin H&E staining showed that only 16 cases had isolated HD, and the remaining had a combined diagnosis of HAD. Moreover, 12 of these allied disorders were found at the proximal end of the resected bowel. Patients received follow-up for 6-15 months. The bowel function score of the study group was significantly higher than the control group. Fast LDH staining can clearly identify ganglion cells and rapidly diagnose HD and HAD intraoperatively. In addition, this method is helpful for improving patient prognosis.