CD4(+) T cells play a crucial role in the pathogenesis of acquired aplastic anemia (AA). Tcf-1 gene regulates T cell development and function, and it is significantly upregulated in the bone marrow CD4(+) T cells from patients with acquired AA. To explore the role of Tcf-1 in the pathogenesis of AA, we knocked down Tcf-1 gene in CD4(+) T cells of AA patients and studied the effects of Tcf-1 silencing on its downstream gene expression. Upon transfection of psiRNA into marrow CD4(+) T cells from bone marrow of aplastic anemia patients, the expression of Tcf-1 was significantly knocked down; consequently, expressions of c-Myc and CD44 were also significantly reduced. Our results suggest that Tcf-1 may contribute to pathogenesis of AA by regulating downstream gene expression such as c-myc and CD44.
基金：
National Natural Science Foundation of China [30570773]; Specialized Research Fund for the Doctoral Program of Higher Education [200804871044]
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Hosp, Dept Hematol, Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Zheng Miao,Zheng Kai,Zhou Jianfeng,et al.Tcf-1 gene silence suppresses downstream gene expression in CD4+ T cells from bone marrow of aplastic anemia patients[J].ANNALS OF HEMATOLOGY.2012,91(3):353-358.doi:10.1007/s00277-011-1314-0.
APA：
Zheng, Miao,Zheng, Kai,Zhou, Jianfeng,Sun, Hanying,Huang, Lifang&Liu, Wenli.(2012).Tcf-1 gene silence suppresses downstream gene expression in CD4+ T cells from bone marrow of aplastic anemia patients.ANNALS OF HEMATOLOGY,91,(3)
MLA：
Zheng, Miao,et al."Tcf-1 gene silence suppresses downstream gene expression in CD4+ T cells from bone marrow of aplastic anemia patients".ANNALS OF HEMATOLOGY 91..3(2012):353-358
