The effects of connective tissue growth factor (CTGF) gene silencing on the radiosensitivity of glioblastoma cells (GBM) were investigated. The lentivirus-mediated short hairpin RNA (shRNA) expression vector targeting CTGF was constructed and transinfected into U87MG human GBM cell line. The CTGF gene expression in U87MG cells was significantly down-regulated. After irradiation with 6 MV X-rays at a dose rate of 2.5 Gy/min, the clonogenicity, proliferation and migration of U87MG cells were assayed in vitro. The survival, proliferation and migration of U87MG cells were all remarkably inhibited by CTGF silencing (p < 0.05 vs control). Our results demonstrate that CTGF is important for GBM and CTGF gene silencing can be a potential tool to enhance the sensitivity of GBM to radiotherapy.
基金：
National Nature Science Foundation of China [81272780/H1618]; Huazhong University of Science and Technology frontier exploration fund [2013TS151]
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Oncol, Wuhan 430030, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Han Na,Shahveranov Allahverdi,Cheng Yi,et al.Effects of connective tissue growth factor (CTGF) gene silencing on the radiosensitivity of glioblastoma[J].INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE.2014,7(9):2557-2563.
APA：
Han, Na,Shahveranov, Allahverdi,Cheng, Yi,Qin, Kai,Yu, Shi-Ying&Zhang, Meng-Xian.(2014).Effects of connective tissue growth factor (CTGF) gene silencing on the radiosensitivity of glioblastoma.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,7,(9)
MLA：
Han, Na,et al."Effects of connective tissue growth factor (CTGF) gene silencing on the radiosensitivity of glioblastoma".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 7..9(2014):2557-2563
