Background: Lysimachia capillipes Hemsl (Primulaceae), a folk medicinal plant in China, showed significant anti-tumor activity in recent studies. A reliable LC-MS/MS method was developed and validated for the simultaneous determination of capilliposide B and capilliposide C, the major bioactive components in this plant, in rat plasma. Results: Rat plasma and whole blood samples were pretreated with dichlorvos, an esterase inhibitor, minimizing degradation of analytes in biological samples. The method validation was conducted over the curve range of 10.0 to 5000 ng/ml for both analytes. The intra-and inter-day precision and accuracy of the QC samples showed <= 6.1% RSD and 1.3-3.7% relative error. Conclusion: The method was successfully applied to determine the concentrations of capilliposide B and capilliposide C in incurred rat plasma samples, after administration of Lysimachia capillipes Hemsl extract for a rat PK study.
基金：
National Science and Technology Major Project of China [2012ZX09102201-10]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2013]版：
大类|3 区生物
小类|3 区生化研究方法3 区分析化学
最新[2025]版：
大类|4 区医学
小类|4 区生化研究方法4 区分析化学
JCR分区：
出版当年[2012]版：
Q1CHEMISTRY, ANALYTICALQ2BIOCHEMICAL RESEARCH METHODS
最新[2023]版：
Q3BIOCHEMICAL RESEARCH METHODSQ3CHEMISTRY, ANALYTICAL
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Sch Pharm, Wuhan 430030, Hubei, Peoples R China
通讯作者：
通讯机构：[1]Huazhong Univ Sci & Technol, Tongji Sch Pharm, Wuhan 430030, Hubei, Peoples R China[*1]Huazhong Univ Sci & Technol, Tongji Sch Pharm, 13 Hangkong Rd, Wuhan 430030, Hubei, Peoples R China
推荐引用方式(GB/T 7714)：
Cheng Zhongzhe,Zhang Lin,Zhang Yang,et al.Simultaneous determination of capilliposide B and capilliposide C in rat plasma by LC-MS/MS and its application to a PK study[J].BIOANALYSIS.2014,6(7):935-945.doi:10.4155/BIO.13.328.
APA：
Cheng, Zhongzhe,Zhang, Lin,Zhang, Yang,Chen, Guiying&Jiang, Hongliang.(2014).Simultaneous determination of capilliposide B and capilliposide C in rat plasma by LC-MS/MS and its application to a PK study.BIOANALYSIS,6,(7)
MLA：
Cheng, Zhongzhe,et al."Simultaneous determination of capilliposide B and capilliposide C in rat plasma by LC-MS/MS and its application to a PK study".BIOANALYSIS 6..7(2014):935-945
