Adipose-derived stem cells (ASCs) are a set of multi potent stem cells potentially used in cartilage tissue engineering. We hypothesized that the effect of dynamic compression and co-culture with nucleus pulposus cells (NPCs) promotes ASC proliferation and chondrogenic differentiation. A controlled dynamic compression loading device was utilized to stimulate ASCs obtained from Sprague Dawley (SD) rats and identified by flow cytometry. The proliferation index was measured by carboxyfluorescein succinimidyl ester (CFSE) staining. Dynamic compression, as well as co-culture enhanced chondrogenic differentiation of ASCs as indicated by the expression of SOX-9, type-II collagen and aggrecan, which were measured by real-time PCR and Western blot. In our study, we found dynamic compression promoted the proliferation of ASCs and induced its differentiation into NP-like cells. Combination of dynamic compression and coculture showed an additive effect on NP-like cell differentiation. (C) 2014 Elsevier Ltd. All rights reserved.
基金：
National Natural Science Foundation of China [81271347]
第一作者单位：[1]Nanjing Univ, Affiliated Hosp, Nanjing Drum Tower Hosp Nanjing, Nanjing, Jiangsu, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Dai Jun,Wang Huan,Liu Guo,et al.Dynamic compression and co-culture with nucleus pulposus cells promotes proliferation and differentiation of adipose-derived mesenchymal stem cells[J].JOURNAL OF BIOMECHANICS.2014,47(5):966-972.doi:10.1016/j.jbiomech.2014.01.023.
APA：
Dai, Jun,Wang, Huan,Liu, Guo,Xu, Zhanjiang,Li, Feng&Fang, Huang.(2014).Dynamic compression and co-culture with nucleus pulposus cells promotes proliferation and differentiation of adipose-derived mesenchymal stem cells.JOURNAL OF BIOMECHANICS,47,(5)
MLA：
Dai, Jun,et al."Dynamic compression and co-culture with nucleus pulposus cells promotes proliferation and differentiation of adipose-derived mesenchymal stem cells".JOURNAL OF BIOMECHANICS 47..5(2014):966-972
