单位：[1]Department of Infectious Disease, Institute of Infectious Disease, Tongji hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China华中科技大学同济医学院附属同济医院感染病研究所感染科
This study investigated anti-inflammatory effects and possible mechanisms of Chikusetsusaponin IVa (Chi IVa), one of the main bioactive components in saponins from Panacis japonica (SPJ), which is used in traditional Tujia and Hmong Chinese medicine. To this end, changes in the inflammatory profiles of lipopolysacchride (LPS)-stimulated phrobol 12-myristate 13-acetate(PMA)-differented THP-1 macrophages were evaluated following Chi IVa treatment. The results showed that Chi IVa markedly decreased the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1 beta (IL-1), interleukin-6 (IL-6), and tumor necrosis factor- (TNF-) at both the mRNA and protein level, which proved to be dose-dependent. Further studies revealed that Chi IVa strongly suppressed NF-B activation and downregulated the phosphorylation of ERK, p38, and JNK. Our present study demonstrates that Chi IVa suppresses the production of iNOS, COX-2, IL-1, IL-6, and TNF- in LPS-stimulated THP-1 cells likely by inhibiting NF-B activation and ERK, JNK, and p38 signal pathway phosphorylation.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [NSFC81100282, NSFC81030007, NSFC81171558]; National twelfth 'five years' project in Science and Technology [2013ZX10002003]; Hubei Province's Outstanding Medical Academic Leader Program; Program for Innovative Research Team of Chinese Ministry of Education [IRT1131, IRT14R20]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2013M531700]
第一作者单位：[1]Department of Infectious Disease, Institute of Infectious Disease, Tongji hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China
通讯作者：
通讯机构：[1]Department of Infectious Disease, Institute of Infectious Disease, Tongji hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China[*1]Huazhong Univ Sci & Technol, Dept Infect Dis, Inst Infect Dis, Tongji Hosp,Tongji Med Coll, 1095 Jie Fang Ave, Wuhan 430030, Peoples R China
推荐引用方式(GB/T 7714)：
Wang H.,Qi J.,Li L.,et al.Inhibitory effects of Chikusetsusaponin IVa on lipopolysaccharide-induced pro-inflammatory responses in THP-1 cells[J].INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY.2015,28(3):308-317.doi:10.1177/0394632015589519.
APA：
Wang, H.,Qi, J.,Li, L.,Wu, T.,Wang, Y....&Ning Qin.(2015).Inhibitory effects of Chikusetsusaponin IVa on lipopolysaccharide-induced pro-inflammatory responses in THP-1 cells.INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY,28,(3)
MLA：
Wang, H.,et al."Inhibitory effects of Chikusetsusaponin IVa on lipopolysaccharide-induced pro-inflammatory responses in THP-1 cells".INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY 28..3(2015):308-317
