Background: Thailandepsin B (TDP-B) is a potent histone deacetylase inhibitor under development. A reliable bioanalytical method for the quantification of TDP-B in plasma samples is required. Results: The stabilizer mixture containing hydrochloric acid, formic acid and dichlorvos was applied to stabilize TDP-B in matrix samples. The method validation was conducted over the curve range of 1.00 to 1000 ng/ml. The intrabatch and interbatch precision and accuracy of the quality control samples showed 10.9% relative standard deviation and -6.7% to 15.0% relative error. Moreover, a possible metabolite M of TDP-B was tentatively characterized. Conclusion: A reliable LC-MS/MS method was developed and validated for the quantification of TDP-B and was successfully applied to a rat pharmacokinetic study.
基金：
"3551 Talent Plan" from the East Lake New Technology Development Zone of Wuhan, China
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2014]版：
大类|3 区生物
小类|2 区分析化学3 区生化研究方法
最新[2025]版：
大类|4 区医学
小类|4 区生化研究方法4 区分析化学
JCR分区：
出版当年[2013]版：
Q1CHEMISTRY, ANALYTICALQ2BIOCHEMICAL RESEARCH METHODS
最新[2023]版：
Q3BIOCHEMICAL RESEARCH METHODSQ3CHEMISTRY, ANALYTICAL
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Sch Pharm, Wuhan 430030, Hubei, Peoples R China
通讯作者：
通讯机构：[1]Huazhong Univ Sci & Technol, Tongji Sch Pharm, Wuhan 430030, Hubei, Peoples R China[*1]Huazhong Univ Sci & Technol, Tongji Sch Pharm, 13 Hangkong Rd, Wuhan 430030, Hubei, Peoples R China
推荐引用方式(GB/T 7714)：
Cheng Zhongzhe,Ding Lian,Zhang Yang,et al.Determination of thailandepsin B in rat plasma with a LC-MS/MS method[J].BIOANALYSIS.2015,7(1):39-52.doi:10.4155/bio.14.264.
APA：
Cheng, Zhongzhe,Ding, Lian,Zhang, Yang,Chen, Guiying,Chen, Chang...&Jiang, Hongliang.(2015).Determination of thailandepsin B in rat plasma with a LC-MS/MS method.BIOANALYSIS,7,(1)
MLA：
Cheng, Zhongzhe,et al."Determination of thailandepsin B in rat plasma with a LC-MS/MS method".BIOANALYSIS 7..1(2015):39-52
