OBJECTIVE To find the potential micro-RNA (miRNA) that could determine the fate of prostate cancer stem cells. MATERIALS AND METHODS We compared miRNA expression between our purified CD133(+) prostatic cancer stem cells (PCSCs) and CD133-cells. Sphere formation assay and matrigel-based cell invasion assay were applied to determine the stemness of CD133(+) PCSCs after our manipulation of miRNA using miRNA mimic or miRNA inhibitor. RESULTS In this study, we identified that miR-34C was under-expressed in the purified CD133(+) PCSCs and enforced introduction of miR-34C attenuated the stemness of CD133(+) PCSCs. Clinically, we also observed a negative correlation between miR-34C and CD133. CONCLUSION Our data strongly suggest that miR-34C may play essential role in conferring castration resistance by equilibrating PSCS population. (C) 2016 Elsevier Inc.
通讯机构：[2]Huazhong Univ Sci & Technol,Tongji Med Coll,Tongji Hosp,Dept Urol,Wuhan 430030,Peoples R China[3]Huazhong Univ Sci & Technol,Tongji Med Coll,Tongji Hosp,Dept Obstet & Gynaecol,Wuhan,Peoples R China[4]Huazhong Univ Sci & Technol, Tongji Med Coll, Inst Family Planning Res, Ctr Reprod Med, Wuhan, Peoples R China
推荐引用方式(GB/T 7714)：
Chen Yuan,Rao Qun,Zhang Huiping,et al.miR-34C Disrupts the Stemness of Purified CD133+ Prostatic Cancer Stem Cells[J].UROLOGY.2016,96:doi:10.1016/j.urology.2016.07.021.
APA：
Chen, Yuan,Rao, Qun,Zhang, Huiping,Xu, Hua,Zhang, Cuntai...&Ye, Zhangqun.(2016).miR-34C Disrupts the Stemness of Purified CD133+ Prostatic Cancer Stem Cells.UROLOGY,96,
MLA：
Chen, Yuan,et al."miR-34C Disrupts the Stemness of Purified CD133+ Prostatic Cancer Stem Cells".UROLOGY 96.(2016)
