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YAP and ERK mediated mechanical strain-induced cell cycle progression through RhoA and cytoskeletal dynamics in rat growth plate chondrocytes

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单位： [1]Huazhong Univ Sci & Technol,Dept Orthoped,Tongji Med Coll,Tongji Hosp,Wuhan 430030,Hubei Province,Peoples R China [2]Soochow Univ, Affiliated Hosp 1, Dept Orthoped, Suzhou 215006, Jiangsu, Peoples R China [3]Huazhong Univ Sci & Technol,Dept Rehabil,Tongji Med Coll,Tongji Hosp,Wuhan 430030,Hubei Province,Peoples R China

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关键词： growth plate chondrocytes mechanical strain yes-associated protein (YAP) extracellular signal-regulated kinase (ERK) mechanotransduction

摘要：

Yes-associated protein (YAP) and extracellular signal-regulated kinase (ERK) have been considered as key regulators in tissue homeostasis, organ development, and tumor formation. However, the roles of YAP and ERK in the mediating strain mechanosensing in the growth plate cartilage have not been determined. In this study, chondrocytes obtained from the growth plate cartilage of 2-week-old Sprague-Dawley rats were subjected to the mechanical strain with different magnitudes and durations at a frequency of 0.5Hz. We found that YAP and ERK activation in response to mechanical strain was time and magnitude dependent. Pretreatment with a RhoA inhibitor (C3 toxin) or a microfilament cytoskeleton disrupting reagent (cytochalasin D) could suppress their activation. In addition, activated YAP and ERK were able to induce cell cycle progression by up-regulating the expression of cell cycle-related genes. These results shed new light on the function of YAP and ERK in mechanical strain-promoted growth plate development. Our results also provided evidence that RhoA and cytoskeletal dynamics are required for this mechanotransduction. (c) 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1121-1129, 2016.

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出版当年[2015]版：

大类 | 3 区医学

小类 | 2 区骨科

最新[2025]版：

大类 | 3 区医学

小类 | 4 区骨科

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出版当年[2014]版：

Q1 ORTHOPEDICS

最新[2023]版：

Q2 ORTHOPEDICS

影响因子： 2.1 最新[2023版] 2.9 最新五年平均 2.986 出版当年[2014版] 3.221 出版当年五年平均 2.972 出版前一年[2013版] 2.807 出版后一年[2015版]

第一作者：

第一作者单位： [1]Huazhong Univ Sci & Technol,Dept Orthoped,Tongji Med Coll,Tongji Hosp,Wuhan 430030,Hubei Province,Peoples R China [2]Soochow Univ, Affiliated Hosp 1, Dept Orthoped, Suzhou 215006, Jiangsu, Peoples R China

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