Absent in melanoma 2 (AIM2) inflammasome is a multiprotein complex which plays a pivotal role in the host immune response to multiple pathogens. The role of AIM2 in human cytomegalovirus (HCMV) infection is poorly studied. Thus, using a small inference RNA (siRNA) approach and THP-1 derived macrophage cells infected with HCMV AD169 strain, we investigated the impact of HCMV infection on AIM2-mediated molecular events. Compared to wild-type cells, AIM2-defiecient macrophages showed a limited ability to activate caspase-1, process IL-1 beta, and induce cell death. In addition, AIM2-defiecient cells were unable to efficiently control HCMV infection, as the transcription of virus DNA polymerase gene UL54 and major tegument protein gene UL83 were higher compared to wild-type cells. In conclusion, HCMV infection induces an AIM2 inflammasome response, which negatively influences viral life cycle.
基金：
National Natural Science Foundation of China [81271807, 81301425]
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Pediat, Wuhan, Hubei, Peoples R China
通讯作者：
通讯机构：[1]Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Pediat, Wuhan, Hubei, Peoples R China[*1]Tongji Hosp, Teaching & Res Off Pediat, Jiefang Rd 1095, Wuhan 430030, Hubei, Peoples R China
推荐引用方式(GB/T 7714)：
Huang Yuan,Liu Lingling,Ma Di,et al.Human cytomegalovirus triggers the assembly of AIM2 inflammasome in THP-1-derived macrophages[J].JOURNAL OF MEDICAL VIROLOGY.2017,89(12):2188-2195.doi:10.1002/jmv.24846.
APA：
Huang, Yuan,Liu, Lingling,Ma, Di,Liao, Yi,Lu, Yuanyuan...&Fang, Feng.(2017).Human cytomegalovirus triggers the assembly of AIM2 inflammasome in THP-1-derived macrophages.JOURNAL OF MEDICAL VIROLOGY,89,(12)
MLA：
Huang, Yuan,et al."Human cytomegalovirus triggers the assembly of AIM2 inflammasome in THP-1-derived macrophages".JOURNAL OF MEDICAL VIROLOGY 89..12(2017):2188-2195
