To investigate the effect of dihydrotestosterone (DHT) combined with Dezawa's method on the differentiation of bone marrow stromal cells (BMSCs) into schwann-like cells. Compared to the Dezawa's method, schwann-like cells obtained from our modified method were longer and thinner and exhibited a typical bipolar or tripolar shape. These cells had a higher mRNA expression of S100 and myelin protein zero (P0), about 1.7- and 2.5-fold respectively, while the glial fibrillary acidic protein (GFAP) mRNA level was decreased about 92 %. No significant difference in peripheral myelin protein 22 (PMP22) mRNA expression was found. Immunofluorescence and Western blot showed the similar results. DHT in combination with Dezawa's method to induce a BMSCs to differentiate into schwann-like cells with higher expression of P0, which might be more effective in clinical application than previous method for nerve regeneration.
基金：
National Natural Science Foundation of China [81471270]
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Med Coll, Wuhan Union Hosp, Dept Hand Surg, Wuhan 430022, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Yang Xiaofan,Chen Jianghai,Xue Pingping,et al.Differentiation of bone marrow stromal cells into schwann-like cells using dihydrotestosterone combined with a classical induction method[J].BIOTECHNOLOGY LETTERS.2017,39(2):331-337.doi:10.1007/s10529-016-2239-4.
APA：
Yang, Xiaofan,Chen, Jianghai,Xue, Pingping,Liu, Ranran,Ji, Wei...&Chen, Zhenbing.(2017).Differentiation of bone marrow stromal cells into schwann-like cells using dihydrotestosterone combined with a classical induction method.BIOTECHNOLOGY LETTERS,39,(2)
MLA：
Yang, Xiaofan,et al."Differentiation of bone marrow stromal cells into schwann-like cells using dihydrotestosterone combined with a classical induction method".BIOTECHNOLOGY LETTERS 39..2(2017):331-337
