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The Effects of IL10 and NK Cells on the Susceptibility to Mouse Cytomegalovirus in BALB/c Mice despite the Compensation of IFNγ

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单位： [1]Huazhong Univ Sci & Technol, Tongji Hosp, Dept Pediat, Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China

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关键词： Mouse cytomegalovirus Spleen NK cells IL10 IFN gamma

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Objective: The aim of this study was to determine which factors lead to the susceptibility to mouse cytomegalovirus (MCMV) in the spleens of BALB/c mice. Methods: BALB/c and C57BL/6 mice were randomly divided into a control group and an infection group and sacrificed on day 0, 1, 3, 7, 14, and 28 postinfection. The cytotoxicity of NK cells was determined by evaluating lactate dehydrogenase contents. Flow cytometry was used to analyze activated NK cells, IFN gamma(+) NK cells, and total NK cells in the spleen. The pathological changes of spleens in each group were analyzed by HE staining. The expression of IL10, IL18, IFN gamma, Thpok, and IFN beta of spleens was determined by quantitative reverse transcriptase PCR. The viral loads of MCMV in spleens and salivary glands were also detected. Results: We found that spleen NK cells and IL10 in C57BL/6 mice possessed more powerful immunity to MCMV than BALB/c mice. In BALB/c mice, combined effects of the cytotoxicity of NK cells and IFN gamma in spleens still ended up with deficient control of infection. Conclusion: The functional shortage of NK cells and inappropriate expression of IL10 result in the susceptibility to MCMV in BALB/c mice. (C) 2018 S. Karger AG, Basel

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出版当年[2017]版：

大类 | 4 区医学

小类 | 4 区病毒学

最新[2025]版：

大类 | 4 区医学

小类 | 4 区病毒学

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出版当年[2016]版：

Q4 VIROLOGY

最新[2023]版：

Q3 VIROLOGY

影响因子： 3.2 最新[2023版] 2.5 最新五年平均 1.292 出版当年[2016版] 1.476 出版当年五年平均 1.822 出版前一年[2015版] 1.011 出版后一年[2017版]

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第一作者单位： [1]Huazhong Univ Sci & Technol, Tongji Hosp, Dept Pediat, Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China

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