单位：[1]Huazhong Univ Sci & Technol,Tongji Hosp,Dept Hematol,Tongji Med Coll,Wuhan 430030,Hubei,Peoples R China内科学系血液内科华中科技大学同济医学院附属同济医院[2]Huazhong Univ Sci & Technol,Tongji Hosp,Tongji Med Coll,Canc Biol Res Ctr,Wuhan 430030,Hubei,Peoples R China肿瘤生物医学中心华中科技大学同济医学院附属同济医院妇产科学系妇科肿瘤
The 11q23 of the mixed lineage leukemia 1 (MLL1) gene plays a crucial role in early embryonic development and hematopoiesis. The MLL-AF9 fusion gene, resulting from chromosomal translocation, often leads to acute myeloid leukemia with poor prognosis. Here, we generated a zebrafish model expressing the human MLL-AF9 fusion gene. Microinjection of human MLL-AF9 mRNA into zebrafish embryos resulted in enhanced hematopoiesis and the activation of downstream genes such as meis1 and hox cluster genes. Embryonic MLL-AF9 expression upregulated HSPC and myeloid lineage markers. Doxorubicin and MI-2 (a menin inhibitor) treatments significantly restored normal hematopoiesis in MLL-AF9-expressing animals. This study provides insight into the role of MLL-AF9 in zebrafish hematopoiesis and establishes a robust and efficient in vivo model for high-throughput drug screening.
基金：
National Natural Science Funds of ChinaNational Natural Science Foundation of China (NSFC) [81600125, 81570196, 81630006, 81090414]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|3 区生物
小类|3 区生物工程与应用微生物4 区医学：研究与实验
最新[2025]版：
大类|4 区医学
小类|4 区生物工程与应用微生物4 区医学：研究与实验
JCR分区：
出版当年[2016]版：
Q2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者单位：[1]Huazhong Univ Sci & Technol,Tongji Hosp,Dept Hematol,Tongji Med Coll,Wuhan 430030,Hubei,Peoples R China[2]Huazhong Univ Sci & Technol,Tongji Hosp,Tongji Med Coll,Canc Biol Res Ctr,Wuhan 430030,Hubei,Peoples R China
通讯作者：
通讯机构：[1]Huazhong Univ Sci & Technol,Tongji Hosp,Dept Hematol,Tongji Med Coll,Wuhan 430030,Hubei,Peoples R China[2]Huazhong Univ Sci & Technol,Tongji Hosp,Tongji Med Coll,Canc Biol Res Ctr,Wuhan 430030,Hubei,Peoples R China
推荐引用方式(GB/T 7714)：
Tan Jiaqi,Zhao Lei,Wang Gaoxiang,et al.Human MLL-AF9 Overexpression Induces Aberrant Hematopoietic Expansion in Zebrafish[J].BIOMED RESEARCH INTERNATIONAL.2018,2018:doi:10.1155/2018/6705842.
APA：
Tan, Jiaqi,Zhao, Lei,Wang, Gaoxiang,Li, Tongjuan,Li, Dan...&Zhou, Jianfeng.(2018).Human MLL-AF9 Overexpression Induces Aberrant Hematopoietic Expansion in Zebrafish.BIOMED RESEARCH INTERNATIONAL,2018,
MLA：
Tan, Jiaqi,et al."Human MLL-AF9 Overexpression Induces Aberrant Hematopoietic Expansion in Zebrafish".BIOMED RESEARCH INTERNATIONAL 2018.(2018)
