Embryo implantation is a complicated physiological process tightly regulated by multiple biological molecules including growth factors. Transforming growth factor-betas (TGF-beta s) and their most specific signal transduction factors, Smads, are expressed in the endometrium during the window of implantation. Recent researches indicated that Smad dependent TGF-beta signaling may play an important role in the process of embryo implantation. In this study, we measured the expression of TGF-beta 1, TGF-beta receptor type I (T beta RI), Smad3 and p-Smad3 in the endometrium of mice and observed their elevation on day 4, 5 and 6 of pseudopregnancy. Then we administrated a specific Smad3 inhibitor (Sis3) into the uterine cavity of mice on day 3 of pregnancy. The results showed a reduction in insulin-like growth factor-1 (IGFBP-1) expression and the decreased number of implanted embryo after the administration. In addition, Sis3 was found to reduce the IGFBP-1 secretion in decidualized endometrial stromal cells. Taken all together, our findings demonstrated that TGF-beta/Smad3 signaling is involved in the process of embryo implantation.
基金：
National Natural Science Foundation of China [81701450]
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Reprod Med Ctr, Wuhan 430030, Peoples R China[2]Zhejiang Univ, Sch Med, Womens Hosp, Dept Gynecol, Hangzhou 310000, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Li Juan,Dong Xi-yuan,Yang Pei-wen,et al.Activation of Uterine Smad3 Pathway Is Crucial for Embryo Implantation[J].CURRENT MEDICAL SCIENCE.2019,39(6):997-1002.doi:10.1007/s11596-019-2134-z.
APA：
Li, Juan,Dong, Xi-yuan,Yang, Pei-wen,Yang, Shu-lin,Hu, Dan...&Sui, Cong.(2019).Activation of Uterine Smad3 Pathway Is Crucial for Embryo Implantation.CURRENT MEDICAL SCIENCE,39,(6)
MLA：
Li, Juan,et al."Activation of Uterine Smad3 Pathway Is Crucial for Embryo Implantation".CURRENT MEDICAL SCIENCE 39..6(2019):997-1002
