Gene therapy targeted human papillomavirus (HPV) is a promising treatment for cervical cancer, and the key for clinical application depends on an effective gene delivery method. Our aim was to formulate a new pharmaceutical formula for appropriate gene delivery intravaginally. For the first time, we here developed a new polyethylenimine (PEI) based vaginal suppository. The sectional immunofluorescence results confirmed the delivery efficacy both in vivo and in vitro. The quenching fluorescence and decreased gene expression in topical epithelium of green fluorescence protein (GFP) transgenic mice demonstrated the efficient targeting potential of the suppository. The other aim of this study was to evaluate the biocompatibility of the PEI based transfer. To our knowledge, this was also the first study to explore the toxicity in vivo systematically and comprehensively. Our study provided novel ideas for the translational application of PEI based suppository to the prevention and treatment of cervical cancer. (C) 2019 The Author(s). Published by Elsevier Inc.
基金：
National Natural Science Funding of ChinaNational Natural Science Foundation of China (NSFC) [81772786, 81502252, 81630060]; National Key Research and Development Program of China [81830074]
基金编号：81772786 81502252 8163006081830074
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|2 区医学
小类|2 区医学：研究与实验3 区纳米科技
最新[2025]版：
大类|3 区医学
小类|3 区医学：研究与实验3 区纳米科技
JCR分区：
出版当年[2017]版：
Q1NANOSCIENCE & NANOTECHNOLOGYQ1MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q2MEDICINE, RESEARCH & EXPERIMENTALQ2NANOSCIENCE & NANOTECHNOLOGY
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Obstet & Gynecol, Wuhan 430030, Hubei, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
ren ci,li xiaomin,mao lu,et al.An effective and biocompatible polyethylenimine based vaginal suppository for gene delivery[J].NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE.2019,20:doi:10.1016/j.nano.2019.03.016.
APA：
ren,ci,li,xiaomin,mao,lu,xiong,jinfeng,gao,chun...&wang,hui.(2019).An effective and biocompatible polyethylenimine based vaginal suppository for gene delivery.NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE,20,
MLA：
ren,ci,et al."An effective and biocompatible polyethylenimine based vaginal suppository for gene delivery".NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE 20.(2019)
