Protein kinase CK2 is a ubiquitously expressed kinase in eukaryotes, which is known to phosphorylate many protein substrates. Because CK2 is involved in the regulation of various signaling pathways, we wondered whether CK2 participated in the regulation of ionizing radiation (IR) induced biological process. In this study, we investigated the effect of IR on the subcellular localization and kinase activity in human non-small cell lung cancer (NSCLC) cells. Immunofluorescent results showed that CK2 subunits shuttle into the nucleus mostly beginning 1 h after IR and lasting more than 6 h. We also conducted in vitro kinase assay and observed an increase in CK2 kinase activity at 6 h after IR. Furthermore, an increase in S phase was observed at 6 h after IR. Colony formation assay results demonstrated that CK2 inhibitor CX-4945 significantly enhanced the effect of irradiation in NSCLC cells. These results indicated that CK2 may be implicated in the regulation of IR-induced biological process.
基金：
National Nature Science Foundation of China for Young Scholar [NSFC] [81101690]; Natural Science Foundation of Hubei Province of China [2016cfb426]; Wuhan Key Talent Training Project; Applied Basic Research Foundation of Wuhan Science and Technology Committee [2014060101010034]
语种：
外文
被引次数：
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PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|4 区医学
小类|2 区核科学技术3 区生物学4 区核医学
最新[2025]版：
大类|4 区医学
小类|3 区核科学技术4 区生物学4 区核医学
JCR分区：
出版当年[2017]版：
Q1NUCLEAR SCIENCE & TECHNOLOGYQ2BIOLOGYQ3RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
最新[2023]版：
Q1NUCLEAR SCIENCE & TECHNOLOGYQ2BIOLOGYQ2RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Canc Ctr, Wuhan 430022, Hubei, Peoples R China
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推荐引用方式(GB/T 7714)：
Li Qianwen,Li Ke,Zhang Sheng,et al.The effect of ionizing radiation on the subcellular localization and kinase activity of protein kinase CK2 in human non-small cell lung cancer cells[J].INTERNATIONAL JOURNAL OF RADIATION BIOLOGY.2019,95(11):1462-1471.doi:10.1080/09553002.2019.1642531.
APA：
Li, Qianwen,Li, Ke,Zhang, Sheng,Zhou, Yu,Hong, Jiaxin...&Meng, Rui.(2019).The effect of ionizing radiation on the subcellular localization and kinase activity of protein kinase CK2 in human non-small cell lung cancer cells.INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,95,(11)
MLA：
Li, Qianwen,et al."The effect of ionizing radiation on the subcellular localization and kinase activity of protein kinase CK2 in human non-small cell lung cancer cells".INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 95..11(2019):1462-1471
