The aim of this retrospective study was to demonstrate the association of HPV infection and pregnancy, delivery and neonatal outcome in women undergoing artificial insemination by donor (AID). From July 2015 to June 2016 in Tongji Reproductive Medicine Center, 329 women undergoing AID were divided into two sub-groups, HPV positive group and HPV negative group. All the couples were followed up to determine pregnancy, delivery and neonatal outcome. Forty-four (13.4%) women was detected HPV infection. HPV infection could reduce clinical pregnancy rate and live birth rate significantly of women undergoing AID (P=0.008; P=0.008). However, for miscarriage rate, there was no significant difference (0.6% vs. 2.7%; P=0.446). There were no significant differences of pregnancy outcome among various HPV type groups. Moreover, we found that only gestational weeks showed significant difference between two groups (36.00 +/- 0.71 vs. 38.74 +/- 1.65; P=0.023). No difference was noted in neonatal weight (5.10 +/- 0.14 vs. 6.45 +/- 1.01; P=0.066), neonatal sex (P=0.490), delivery mode (P=0.655). The infection of HPV could reduce the clinical pregnancy rate and live birth rate, without improving miscarriage rate. Pregnant HPV exposure for the women undergoing AID could short the gestational weeks.