Matrix stiffness is known to alter cellular behaviors in various biological contexts. Previous investigations have shown that epithelial mesenchymal transition (EMT) promotes the progression and invasion of tumor. Mechanical signaling is identified as a regulator of EMT. However, the molecular mechanisms underlying the influence exerted by matrix stiffness on EMT in osteosarcoma remains largely unknown. Using polyacrylamide hydrogel model, we investigate the effects of matrix stiffness on EMT and migration in osteosarcoma. Our data indicates that high matrix stiffness regulates cell morphology and promotes EMT and migration in osteosarcoma MG63 cell line in vitro. Notably, matrix stiffness promotes polymerization of actin and nuclear accumulation of myocardin-related transcription factor A (MRTF-A). Furthermore, inhibiting MRTF-A by CCG 203971 significantly reduces EMT and migration on rigid gels. These data suggest that matrix stiffness of the tumor microenvironment actively regulate osteosarcoma EMT and migration through cytoskeletal remodeling and translocation of MRTF-A, which may contribute to cancer progression.
第一作者单位：[1]Huazhong Univ Sci & Technol,Tongji Med Coll,Dept Orthoped,Tongji Hosp,1095 Jiefang Ave,Wuhan 430030,Hubei,Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Dai Jun,Qin Liang,Chen Yan,et al.Matrix stiffness regulates epithelial-mesenchymal transition via cytoskeletal remodeling and MRTF-A translocation in osteosarcoma cells[J].JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS.2019,90:226-238.doi:10.1016/j.jmbbm.2018.10.012.
APA：
Dai, Jun,Qin, Liang,Chen, Yan,Wang, Huan,Lin, Guanlin...&Fang, Huang.(2019).Matrix stiffness regulates epithelial-mesenchymal transition via cytoskeletal remodeling and MRTF-A translocation in osteosarcoma cells.JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS,90,
MLA：
Dai, Jun,et al."Matrix stiffness regulates epithelial-mesenchymal transition via cytoskeletal remodeling and MRTF-A translocation in osteosarcoma cells".JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS 90.(2019):226-238
