单位：[1]Chinese Acad Med Sci, Natl Hlth Commiss Peoples Republ China, Peking Union Med Coll Hosp, Dept Endocrinol,Key Lab Endocrinol,Peking Union M, Beijing, Peoples R China[2]Huazhong Univ Sci Technol, Dept Endocrinol, Tongji Hosp, Tongji Med Coll, Wuhan, Peoples R China内科学系内分泌内科华中科技大学同济医学院附属同济医院[3]Chinese Acad Med Sci, Dept Urol, Peking Union Med Coll Hosp, Peking Union Med Coll, Beijing, Peoples R China
Purpose: The aim of this study is to examine the effects of different kinds of calcium channel blockers (CCBs) on primary aldosterone-producing adenoma (APA) mainly with KCNJ5 mutations. Primary cultured APA cells were treated with different calcium channel blockers (L/T type CCB benidipine, T-type CCB mibefradil and L-type CCB nifedipine), and aldosterone secretagogues with or without nifedipine. Aldosterone level, aldosterone synthase (CYP11B2) mRNA expression and cell proliferation were detected. The results showed that all three CCBs significantly inhibit aldosterone secretion and CYP11B2 mRNA expression. Benidipine was relatively more effective than mibefradil or nifedipine. In addition, only mibefradil marginally inhibited cell proliferation. Adrenocorticotropin (ACTH) had a much stronger effect in stimulating aldosterone secretion and promoting cell proliferation from APA's than angiotensin II (ATII). Different from ACTH and ATII, potassium had no effect. Nifedipine inhibited the basal and ACTH-, ATII-elicited aldosterone secretion. Twenty three of 24 APAs had somatic KCNJ5 mutation. In conclusion, benidipine, mibefradil and nifedipine significantly inhibit aldosterone secretion in primary cultured APA cells.
基金：
National Key Program of Clinical Science of China [81770427]; Chinese Academy of Medical Sciences (CAMS) Initiative for Innovative Medicine (CAMS-I2M) [2017-I2M-1-001]
第一作者单位：[1]Chinese Acad Med Sci, Natl Hlth Commiss Peoples Republ China, Peking Union Med Coll Hosp, Dept Endocrinol,Key Lab Endocrinol,Peking Union M, Beijing, Peoples R China[2]Huazhong Univ Sci Technol, Dept Endocrinol, Tongji Hosp, Tongji Med Coll, Wuhan, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Wang Fen,Ma Xiaosen,Tong Anli,et al.The Effects of Different Calcium Channel Blockers on Aldosterone-Producing Adenoma Cells[J].FRONTIERS IN ENDOCRINOLOGY.2020,11:doi:10.3389/fendo.2020.00260.
APA：
Wang, Fen,Ma, Xiaosen,Tong, Anli,Zhang, Yushi,Wen, Jin&Li, Yuxiu.(2020).The Effects of Different Calcium Channel Blockers on Aldosterone-Producing Adenoma Cells.FRONTIERS IN ENDOCRINOLOGY,11,
MLA：
Wang, Fen,et al."The Effects of Different Calcium Channel Blockers on Aldosterone-Producing Adenoma Cells".FRONTIERS IN ENDOCRINOLOGY 11.(2020)
