Aim To evaluate the antibiofilm effect of proanthocyanidin (PA) solution as an irrigant against Enterococcus faecalis (E. faecalis) and its influence on the mechanical properties and biodegradation resistance of demineralized root dentine. Methodology Enterococcus faecalis were introduced into human root dentine tubules by a serial centrifugation method and grown for 1 week. Dentine blocks infected with 1-week-old E. faecalis biofilms were treated with the following irrigants: sterile water (control), 2% chlorhexidine (CHX), 2% PA, 5% PA and 10% PA. After treatment, the live and dead bacteria proportions within E. faecalis biofilms were analysed using confocal laser scanning microscopy. To evaluate the biostability of fully demineralized dentine treated by the aforementioned irrigants, the elastic modulus and hydroxyproline release of human dentine incubated in collagenase solution were tested at baseline, after irrigant treatment and after biodegradation, respectively. Furthermore, the surface chemical bond of demineralized dentine collagen treated by various irrigants was characterized by X-ray photoelectron spectroscopy (XPS). Statistical analysis was performed using one-way anova and Tukey's post hoc multiple comparisons with the significance level at 5%. Results The proportion of dead E. faecalis volume was significantly higher in the PA and CHX groups than that in the control group (P < 0.05). PA irrigation significantly increased the mechanical properties of demineralized dentine (P < 0.05), and the effect was enhanced with increasing PA concentration. CHX and PA groups had significantly less elasticity loss and hydroxyproline release (P < 0.05). The biomodification of dentine collagen by PA was verified by increased C-O/C-N peak percentage under C1s and C-O peak percentage under O1s narrow-scan XPS spectra. Conclusions Proanthocyanidin killed E. faecalis within biofilms and enhanced the biostability of the collagen matrix of demineralized root dentine. It might be used as an auxiliary endodontic irrigant with antibiofilm and collagen-stabilizing effects.
基金：
National Natural Science Foundation of China [81401525, 81871492, 81571815, 81771121] Funding Source: Medline
第一作者单位：[1]Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Ctr Stomatol, 1095 Jiefang Ave, Wuhan 430030, Peoples R China[2]Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Shanghai Key Lab Stomatol,Dept Oral Surg, Shanghai, Peoples R China[3]Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Shanghai Res Inst Stomatol,Dept Oral Surg, Shanghai, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Yang S. Y.,Liu Y.,Mao J.,et al.The antibiofilm and collagen-stabilizing effects of proanthocyanidin as an auxiliary endodontic irrigant[J].INTERNATIONAL ENDODONTIC JOURNAL.2020,53(6):824-833.doi:10.1111/iej.13280.
APA：
Yang, S. Y.,Liu, Y.,Mao, J.,Wu, Y. B.,Deng, Y. L....&Gong, S. Q..(2020).The antibiofilm and collagen-stabilizing effects of proanthocyanidin as an auxiliary endodontic irrigant.INTERNATIONAL ENDODONTIC JOURNAL,53,(6)
MLA：
Yang, S. Y.,et al."The antibiofilm and collagen-stabilizing effects of proanthocyanidin as an auxiliary endodontic irrigant".INTERNATIONAL ENDODONTIC JOURNAL 53..6(2020):824-833
医学