OBJECTIVE. The objective of our study was to compare the feasibility of R2* and apparent diffusion coefficient (ADC) for differentiating tumor grade and T category of bladder cancer. SUBJECTS AND METHODS. In this prospective study, 58 patients with pathologically confirmed bladder cancers underwent pretreatment T2*-weighted imaging and DWI on a 3-T MRI unit. The apparent transverse relaxation rate R2*, which is derived from T2*weighted imaging, and ADC, which is derived from DWI, were calculated and compared between low- and high-grade bladder cancers as well as between non-muscle-invasive bladder cancers (NMIBCs) and muscle-invasive bladder cancers (MIBCs) using the Mann-Whitney U test. The diagnostic performances of R2*, ADC, and the combination of R2* and ADC were evaluated through an ROC analysis. RESULTS. Significant differences were found in R2* (mean +/- SD, 16.55 +/- 5.54 vs 20.96 +/- 7.75 s(-1);p = 0.000 and ADC (1.62 +/- 0.31 vs 1.33 +/- 0.21 x 10(-3) mm(2)/s; p < 0.001) between lowand high-grade bladder cancers. R2* was significantly higher (22.56 +/- 8.41 vs 18.06 +/- 6.46 s(-1);p = 0.008) and ADC was considerably lower (1.21 +/- 0.18 vs 1.53 +/- 0.27 x 10(-3) mm(2)/s;p < 0.001) in MIBCs than in NMI BO. The AUCs for differentiating low- from high-grade groups were 0.714 using R2* and 0.779 using ADC. AUCs for distinguishing between NMIBC and MIBC groups using R2* and ADC were 0.682 and 0.850, respectively. CONCLUSION. In addition to ADC, R2* can be used as a quantitative imaging biomarker to provide additional information for tumor characterization of bladder cancer.