单位：[1]Department of Orthopedics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China外科学系华中科技大学同济医学院附属同济医院骨科[2]Orthopedic Hospital, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang 330006, China[3]Anhui University of Science and Technology Institute of Precision Medicine (AUST-IPM), Huainan 232001, China[4]Research and Clinical Translation Center of Gene Medicine and Tissue Engineering, School of Public Health, Anhui University of Science and Technology, Huainan 232001, China[5]Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, China[6]State Key Laboratory of Materials Processing and Die & Mould Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China
Cartilage has limited self-repair ability, leading to osteoarthritis postinjury and ultimately physical disability in people. In addition, cartilage injury is accompanied by loss of extracellular matrix (ECM) and infiltration of inflammation, which makes cartilage regeneration more difficult. In this study, a hybrid hydrogel of hydroxypropyl chitin and oxidized chondroitin sulfate (HPCH-OCS) with injectable and thermosensitive properties was prepared by a Schiff base reaction. In vitro, the HPCH-OCS hydrogel showed desirable biocompatibility and bioactivity by promoting chondrogenic differentiation of stem cells, maintaining the chondrocyte phenotype, and inhibiting proteins related to cartilage ECM catabolism under inflammatory conditions. In vivo, the HPCH-OCS hydrogel combined with microfracture could effectively repair cartilage defects and promote cartilage regeneration. In conclusion, the HPCH-OCS hydrogel has good biological and mechanical properties, and its combination with microfracture provides an effective strategy for clinical cartilage regeneration.
基金：
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing (Wuhan University of Technology) [2022-KF-29]; Natural Science Foundation of China [82202673, 81974350]; Fundamental Research Funds for the Central Universities [HUST: 2022JYCXJJ049]
第一作者单位：[1]Department of Orthopedics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China[2]Orthopedic Hospital, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang 330006, China
材料科学