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Multiscale imaging of corneal endothelium damage and Rho-kinase inhibitor application in mouse models of acute ocular hypertension

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单位： [1]Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA. [2]Currently with Department of Ophthalmology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,China. [3]Currently with Program of Polymer and Color Chemistry, Department of Textile Engineering, Chemistry and Science, North Carolina State University, Raleigh, NC 27606, USA. [4]Department of Mechanical Engineering, Northwestern University, Evanston, IL 60208, USA. [5]Spencer Center for Vision Research, Byers Eye Institute, Department of Ophthalmology, Stanford University, Palo Alto, CA 94303, USA.

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We developed a multiscale optical imaging workflow, integrating and correlating visible-light optical coherence tomography, confocal laser scanning microscopy, and single-molecule localization microscopy to investigate mouse cornea damage from the in-vivo tissue level to the nanoscopic single-molecule level. We used electron microscopy to validate the imaged nanoscopic structures. We imaged wild-type mice and mice with acute ocular hypertension and examined the effects of Rho-kinase inhibitor application. We defined four types of intercellular tight junction structures as healthy, compact, partially-distorted, and fully-distorted types by labeling the zonula occludens-1 protein in the corneal endothelial cell layer. We correlated the statistics of the four types of tight junction structures with cornea thickness and intraocular pressure. We found that the population of fully-distorted tight junctions correlated well with the level of corneal edema, and applying Rho-kinase inhibitor reduced the population of fully-distorted tight junctions under acute ocular hypertension. Together, these data point to the utility of multiscale optical imaging in revealing fundamental biology relevant to disease and therapeutics.© 2024 Optica Publishing Group.

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出版当年[2023]版：

大类 | 2 区医学

小类 | 2 区生化研究方法 2 区光学 2 区核医学

最新[2025]版：

大类 | 3 区医学

小类 | 2 区生化研究方法 3 区光学 3 区核医学

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出版当年[2022]版：

Q2 BIOCHEMICAL RESEARCH METHODS Q2 OPTICS Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

最新[2023]版：

Q2 BIOCHEMICAL RESEARCH METHODS Q2 OPTICS Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

影响因子： 2.9 最新[2023版] 3.2 最新五年平均 3.4 出版当年[2022版] 3.7 出版当年五年平均 3.562 出版前一年[2021版] 2.9 出版后一年[2023版]

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第一作者单位： [1]Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA. [2]Currently with Department of Ophthalmology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,China.

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