Bioadhesive hydrogels have offered unprecedented opportunities in hemostatic agents and tissue sealing, however the application of existing bioadhesive hydrogels through narrow spaces to achieve strong adhesion in fluid-rich physiological environments is challenged either by undesired indiscriminate adhesion or weak wet tissue adhesion. Here we propose a laparoscopically compatible asymmetric adhesive hydrogel (aAH) composed of sprayable adhesive hydrogel powders and injectable anti-adhesive glue to hemostasis and seal the bloody tissues in a non-pressing way, allowing for preventing postoperative adhesion. The powders can seed on the irregular bloody wound to rapidly absorb interfacial fluid, crosslink, and form an adhesive hydrogel to hemostatic seal (blood clotting time and tissue sealing in 10 s, approximately 200 mmHg of burst pressure in sealed porcine tissues). The aAH can be simply formed by crosslinking the upper powder with injectable glue to prevent postoperative adhesion (adhesive strength as low as 1 kPa). The aAH outperforms commercial hemostatic agents and sealants in the sealing of bleeding organs in live rats, demonstrating superior anti-adhesive efficiency. Furthermore, the hemostatic seamless sealing by aAH succeeds in shortening the time of warm ischemia, decreasing the blood loss and reducing the possibility of rebleeding in the porcine laparoscopic partial nephrectomy model. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.