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Microsatellite markers for genotyping of Kodamaea ohmeri: Demonstrating outbreaks based on a multicenter surveillance study in China

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收录情况： ◇ SCIE

作者：
Li Yanbing[1,2,3] * ; Huang Yuyan[1,2,3] ; Kang Mei[4] ; Chen Xiangyang[5] ; Liu Liwen[6] ; Zhao Hongmei[6] ; Chen Zhongju[7] ; Xiao Meng[1,2] ; Xu Yingchun[1,2] ; Yi Qiaolian[1,2,*1] ; Zhou Menglan[1,2,*1] ;
单位： [1]Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China [2]Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China [3]Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China [4]Department of Laboratory Medicine, West China hospital Sichuan University, China [5]The Fifth Clinical Medical College of Henan University of Chinese Medicine (Zhengzhou People’s Hospital), China [6]Department of Laboratory Medicine, The People’s Hospital of Liaoning Province, Shenyang, China [7]Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China
出处：
DOI： 10.1016/j.meegid.2023.105547
ISSN：

关键词： Microsatellite Kodamaea ohmeri Outbreaks

摘要：
Kodamaea ohmeri, an emerging human pathogen, caused both sporadic and nosocomial infections among immunocompromised people with high mortality. However, there is limited research on the molecular epidemiology of K. ohmeri. A total of fifty microsatellite loci were designed based on K. ohmeri type strain NRRL Y-1932 and three loci were finally selected for microsatellite analysis. Non-duplicated K. ohmeri isolates and strains of other species were collected across China as a part of CHIF-NET program for sensitivity and specificity verification. Antifungal susceptibility was determined using Sensititre YeastOne TM YO10. The three loci (P10, P11 and P26), with a cumulative discriminatory power of 0.98, exhibited a prospective specificity and reproducibility in the PCR of 92 K. ohmeri strains from different hospitals. A total of 54 microsatellite types (MT) were identified and most of them distributed sporadically. However, six strains of MT12 clustered in HZ hospital and were isolated in the same department within two months, indicating a potential outbreak. Of seven isolates exhibited MIC values of >8 mg/L for fluconazole, three isolates from LR hospital shared the same genotype of MT44. Herein, we established a set of microsatellite loci for K. ohmeri, as a rapid and specific tool for genotyping K. ohmeri, and identified several potential clusters. This study will help us better understand the molecular epidemiology of the emerging pathogen K. ohmeri.Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.

基金：
This work was supported by the National High Level Hospital Clin- ical Research Funding [2022-PUMCH-B-074]; National Science Foun- dation for Young Scientists of China [82202541] and Fundamental Research Funds for the Central Universities [3332022012].
语种：
外文
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PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类 | 4 区 医学
小类 | 4 区 传染病学
最新[2025]版：
大类 | 4 区 医学
小类 | 4 区 传染病学
JCR分区：
出版当年[2022]版：
Q3 INFECTIOUS DISEASES
最新[2023]版：
Q3 INFECTIOUS DISEASES

影响因子： 最新[2023版] 最新五年平均 出版当年[2022版] 出版当年五年平均 出版前一年[2021版] 出版后一年[2023版]

第一作者：
第一作者单位： [1]Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China [2]Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China [3]Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China
通讯作者：
通讯机构： [1]Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China [2]Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China [*1]partment of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China
推荐引用方式(GB/T 7714)：
Li Yanbing,Huang Yuyan,Kang Mei,et al.Microsatellite markers for genotyping of Kodamaea ohmeri: Demonstrating outbreaks based on a multicenter surveillance study in China[J].INFECTION GENETICS AND EVOLUTION.2024,117:105547.doi:10.1016/j.meegid.2023.105547.
APA：
Li Yanbing,Huang Yuyan,Kang Mei,Chen Xiangyang,Liu Liwen...&Zhou Menglan.(2024).Microsatellite markers for genotyping of Kodamaea ohmeri: Demonstrating outbreaks based on a multicenter surveillance study in China.INFECTION GENETICS AND EVOLUTION,117,
MLA：
Li Yanbing,et al."Microsatellite markers for genotyping of Kodamaea ohmeri: Demonstrating outbreaks based on a multicenter surveillance study in China".INFECTION GENETICS AND EVOLUTION 117.(2024):105547

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