Background: We aimed to explore the effect of humanistic care time checklist on nursing quality of intensive care unit (ICU) patients. Methods: Overall, 230 patients hospitalized in ICU of Tongji Medical College of Huazhong University of Sci-ence and Technology, Wuhan, China from June 2018 to May 2019 were included in this retrospective study . Overall, 115 patients were included in the control group. Another 115 patients were included in the observation group. The nurses communicated routinely with the patients in the control group, and communicated with the patients in the observation group according to the self-designed humanistic care time checklist. The results of the implementation of humanistic care measures were evaluated by "ICU patients' humanistic care time satisfac-tion questionnaire". Results: The satisfaction of humanistic care in the observation group was significantly higher than that in the control group (P<0.05). Conclusion: Communication between nurses and patients according to the requirements of humanistic care time checklist can improve patients' satisfaction with nursing care.