In the current study, Allopurinol was loaded into chitosan hydrogel to developed an injectable wound dressing material for treating skin wounds after fracture surgery. The hydrogel system was prepared by cross-linking with Tripolyphosphate. Various in vitro experiments including cell viability assay, cytoprotection assay, cell migra-tion assay, water uptake capacity measurement, and drug release assay were performed to characterize the dressings. In vivo study was performed in a rat modl of excisional wound. Results showed that Allopurinol-IP: 8.46.247.10 On: Wed, 22 Feb 2023 11:26:52 incorporated hydrogel system was not toxic and preserved cell viabity under H2O2-induced oxidative stress. Copyright: American Scientific Publishers Delivered by Ingenta In vivo study revealed that Allopurinol-delivering hydrogel had significantly higher wound contraction than pure chitosan hydrogel and negative control groups. Histopathological studies showed that wounds treated chitosan/ Allopurinol hydrogel had higher collagen deposition and epithelial thickness. This system can be potential applied in the clinic to promote skin wound healing after fracture surgeries.