单位：[1]Department of Anesthesiology,Hubei Key Laboratory of Geriatric Anesthesia and Perioperative Brain Health,and Wuhan Clinical Research Center for Geriatric Anesthesia,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,China,麻醉科华中科技大学同济医学院附属同济医院综合医疗科[2]Department of Orthopedics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,China华中科技大学同济医学院附属同济医院外科学系骨科
Objective: To investigate the role of gut microbiota and metabolites in POCD in elderly orthopedic patients, and screen the preoperative diagnostic indicators of gut microbiota in elderly POCD.Method: 40 elderly patients undergoing orthopedic surgery were enrolled and divided into Control group and POCD group following neuropsychological assessments. Gut microbiota was determined by 16S rRNA MiSeq sequencing, and metabolomics of GC-MS and LC-MS was used to screen the differential metabolites. We then analyzed the pathways enriched by metabolites.Result: There was no difference in alpha or beta diversity between Control group and POCD group. There were significant differences in 39 ASV and 20 genera bacterium in the relative abundance. Significant diagnostic efficiency analyzed by the ROC curves were found in 6 genera bacterium. Differential metabolites in the two groups including acetic acid, arachidic acid, pyrophosphate etc. were screened out and enriched to certain metabolic pathways which impacted the cognition function profoundly.Conclusion: Gut microbiota disorders exist preoperatively in the elderly POCD patients, by which there could be a chance to predict the susceptible population.
基金：
National Natural Science Foundation of China [82001161]; Braun Foundation for Scientific Research in Anesthesia [BBDF-2019-011]
第一作者单位：[1]Department of Anesthesiology,Hubei Key Laboratory of Geriatric Anesthesia and Perioperative Brain Health,and Wuhan Clinical Research Center for Geriatric Anesthesia,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,China,
通讯作者：
推荐引用方式(GB/T 7714)：
Bi Jiangjiang,Xu Yifan,Li Shiyong,et al.Contribution of preoperative gut microbiota in postoperative neurocognitive dysfunction in elderly patients undergoing orthopedic surgery[J].FRONTIERS IN AGING NEUROSCIENCE.2023,15:doi:10.3389/fnagi.2023.1108205.
APA：
Bi, Jiangjiang,Xu, Yifan,Li, Shiyong,Zhan, Gaofeng,Hua, Dongyu...&Luo, Ailin.(2023).Contribution of preoperative gut microbiota in postoperative neurocognitive dysfunction in elderly patients undergoing orthopedic surgery.FRONTIERS IN AGING NEUROSCIENCE,15,
MLA：
Bi, Jiangjiang,et al."Contribution of preoperative gut microbiota in postoperative neurocognitive dysfunction in elderly patients undergoing orthopedic surgery".FRONTIERS IN AGING NEUROSCIENCE 15.(2023)
