单位：[1]Department of Neurosurgery, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441021, China[2]Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China外科学系神经外科华中科技大学同济医学院附属同济医院[3]Department of Physiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China[4]Department of Neurosurgery, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China
ObjectiveTo construct and verificate an RNA-binding protein (RBP)-associated prognostic model for gliomas using integrated bioinformatics analysis.MethodsRNA-sequencing and clinic pathological data of glioma patients from The Cancer Genome Atlas (TCGA) database and the Chinese Glioma Genome Atlas database (CGGA) were downloaded. The aberrantly expressed RBPs were investigated between gliomas and normal samples in TCGA database. We then identified prognosis related hub genes and constructed a prognostic model. This model was further validated in the CGGA-693 and CGGA-325 cohorts.ResultsTotally 174 differently expressed genes-encoded RBPs were identified, containing 85 down-regulated and 89 up-regulated genes. We identified five genes-encoded RBPs (ERI1, RPS2, BRCA1, NXT1, and TRIM21) as prognosis related key genes and constructed a prognostic model. Overall survival (OS) analysis revealed that the patients in the high-risk subgroup based on the model were worse than those in the low-risk subgroup. The area under the receiver operator characteristic curve (AUC) of the prognostic model was 0.836 in the TCGA dataset and 0.708 in the CGGA-693 dataset, demonstrating a favorable prognostic model. Survival analyses of the five RBPs in the CGGA-325 cohort validated the findings. A nomogram was constructed based on the five genes and validated in the TCGA cohort, confirming a promising discriminating ability for gliomas.ConclusionThe prognostic model of the five RBPs might serve as an independent prognostic algorithm for gliomas.
基金：
This study was supported by the National Natural Science Foundation of China (No. 82072795).
第一作者单位：[1]Department of Neurosurgery, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441021, China[2]Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
通讯作者：
通讯机构：[2]Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China[4]Department of Neurosurgery, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China
推荐引用方式(GB/T 7714)：
Peng Peng,Chen Zi-rong,Zhang Xiao-lin,et al.Construction and Verification of an RNA-Binding Protein-Associated Prognostic Model for Gliomas[J].CURRENT MEDICAL SCIENCE.2023,43(1):156-165.doi:10.1007/s11596-022-2694-1.
APA：
Peng, Peng,Chen, Zi-rong,Zhang, Xiao-lin,Guo, Dong-sheng,Zhang, Bin...&Wan, Feng.(2023).Construction and Verification of an RNA-Binding Protein-Associated Prognostic Model for Gliomas.CURRENT MEDICAL SCIENCE,43,(1)
MLA：
Peng, Peng,et al."Construction and Verification of an RNA-Binding Protein-Associated Prognostic Model for Gliomas".CURRENT MEDICAL SCIENCE 43..1(2023):156-165
