单位：[1]Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.[2]Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.华中科技大学同济医学院附属同济医院儿科学系[3]The Second College of Clinical Medical Science, China Three Gorges University, Yichang, Hubei, China.[4]Department of Rehabilitation, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Colorectal cancer (CRC) is a common malignant tumor with strong invasiveness. Given the reported involvement of DEP domain-containing protein 1B (DEPDC1B) in the progression of some cancers, its role in CRC was explored in this study. DEPDC1B expression in CRC was assessed based on database and tissue microarray (TMA). In addition, the knockdown and overexpression of DEPDC1B in CRC cell lines were constructed using small hairpin RNA (shRNA) interference. The biological function of DEPDC1B in CRC was evaluated in vitro and in vivo through loss/gain-of-function assays. The results demonstrated that DEPDC1B was highly expressed in CRC. Furthermore, DEPDC1B had the ability to promote CRC proliferation and migration coupled by cell apoptosis. In vivo results showed that DEPDC1B knockdown significantly inhibited the growth of xenograft tumors. Additionally, the results of antibody array indicated increased apoptosis-promoting proteins and decreased apoptosis-inhibiting proteins in DEPDC1B-knockdown CRC cells. In conclusion, DEPDC1B played a key driver role in CRC progression, and inhibition of its expression may be a potential target for precision medicine in CRC.
基金：
National Natural Science Foundation of
China, Grant/Award Numbers: 81401994
第一作者单位：[1]Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.[4]Department of Rehabilitation, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.[*1]Department of Rehabilitation, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Street, Wuhan 430022, China[*2]Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Street, Wuhan 430022, China
推荐引用方式(GB/T 7714)：
Han Fei,Cheng Chao,Xu Qianqian,et al.DEPDC1B promotes colorectal cancer via facilitating cell proliferation and migration while inhibiting apoptosis[J].CELL CYCLE.2023,22(1):131-143.doi:10.1080/15384101.2022.2110439.
APA：
Han Fei,Cheng Chao,Xu Qianqian,Chen Jisong,Yang Zhaohui&Liu Jun.(2023).DEPDC1B promotes colorectal cancer via facilitating cell proliferation and migration while inhibiting apoptosis.CELL CYCLE,22,(1)
MLA：
Han Fei,et al."DEPDC1B promotes colorectal cancer via facilitating cell proliferation and migration while inhibiting apoptosis".CELL CYCLE 22..1(2023):131-143
